Sterilized Grain Bag

Sterilized Grain Bag

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Our best-selling organic sterilized grain spawn bag was formulated by mycologists to maximize the speed and size of your mushroom grow. (All that's required is a spore syringe or liquid culture!)

Each spawn bag contains our proprietary grain blend featuring hydrated, nutrient-rich, organic oat, millet & corn. Bags also include a self-healing injection port for use with a spore or culture syringe plus a 0.2 micron filter patch to block airborne contaminants while providing even gas exchange. (Note: Color of injection port may vary.)

Spawn to bulk ratio

We recommended 1 lb of colonized grain spawn for every 5 lbs of manure or hardwood sterile substrate. We also recommend using 2.5-5ccs per 3 lb injection port bag. (See Inoculation and Colonization below for full details.)

Sterilization

Mass Mycology's grain spawn bags are sterilized in a commercial-grade autoclave. Sterility is verified using biological indicators and temperature sensors.

Innoculation

To inoculate the grain bag, simply inject your spore or culture syringe through the port in a sterile location. We always recommend clean hands and latex or nitrile gloves to reduce the introduction of contaminants.

It is best practice to wait a minimum of 1 week after arrival before injecting grain bags to ensure they were not compromised during transit. Rest assured that your purchase is 100% guaranteed. If your bag was compromised, we will replace it. 

We recommend using 2.5-5ccs per 3lb injection port bag. We do not typically recommend using an entire syringe in one bag. Doing so adds excessive moisture which can create over saturation in the bag. This over-saturation can pool and is prone to being anaerobic and the mycelium will have a hard time colonizing.

Colonization

Allow your bag to colonize in a cool location out of direct sunlight. Temperature for incubation shouldn't exceed 75 degrees F or dip below 55 degrees F. After a couple weeks, you should start to see mycelium growing on the grain. 

You'll notice the white mycelium growing outward from the injection point. When it has grown three to four inches you should, without opening the bag, carefully break up the colonized grain and mix it into the uncolonized grain. This will dramatically speed up the colonization process.

When the bag is completely white with mycelium your spawn is ready to use. This may take a couple weeks to a month or more depending on the species and vigor of your culture.